Kit includes: bottle adapter, bottle adapter nut, filter inlet cap, grid support, vacuum hose barb, tube compression fitting, 47 mm grid, 47 mm 0.22 µm filter membrane, 47 mm 0.45 µm filter membrane, 1/4″ OD x 1/8″ ID ultra chemical resistant, FEP lined Tygon® tubing (3′), 6″ x 6″ box with shrink wrap insert. Includes universal threads designed for 4L or Wheaton bottles. Use with GL-45 bottles requires Opti-Cap® Adapter (sold separately).