• Faster and easier than SPE and liquid-liquid extraction: just load, wait 5 minutes, then elute.
• Effective removal of proteins, phospholipids, and salts.
• Quick concentration step increases sensitivity.
• Easy to automate for high-throughput laboratories.
• Suitable for a wide range of sample matrices and analyte pKa values.

Volume guidelines: Selecting an SLE format with sufficient loading capacity (1 mg sorbent to 1µL diluted sample) is very important because the entire sample volume (including 1:1 dilution in buffer) is absorbed into the diatomaceous earth sorbent. For example, a 100 µL sample should be diluted 1:1 with buffer for a total volume of 200 µL, which requires use of a 200 mg SLE product.