Improve Characterization of Complex Protein Digests

Using Viva Wide Pore HPLC Columns

• Superior resolution—many peaks contain one or two peptides, not three or more.
• Excellent results with highly aqueous mobile phases, compatible with digest matrices.
• Restek-manufactured silica in Restek-manufactured columns.

Protein analyses often incorporate a combination of liquid chromatography and electrospray mass spectrometry. Typically, a protein sample is chemically or enzymatically digested to produce peptides, HPLC/MS is used to resolve and identify the peptides, and this information is used to search protein databases to identify the protein of interest. This type of analysis is now used in many fields, including the bioanalytical and pharmaceutical disciplines.